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The Amazing Spider-Man (1963) #565 - Comic Book DB
A mysterious young woman with ties to Kraven the Hunter stalks Spider-Man relentlessly. Over a period of weeks she observes him at work, tracking him back to his home and learning precisely where that is. Systematically she works to break down his life, and all of those around him.
With his friends targeted by the new Hunter, Peter has no idea that she's out there... until the moment she finally strikes.